SERUM BIOCHEMICAL AND HISTOLOGICAL ANALYSIS OF Heterobranchus bidorsalis JUVENILES EXPOSED TO VARYING PHOTOPERIOD REGIMES

Authors

  • W.O Fawole
  • E.K Ajani
  • K.D Olawepo
  • C.F Idowu
  • O.J Oyedele

Keywords:

Photoperiod, Heterobranchus bidorsalis, serum biochemistry, histological parameters

Abstract

This study investigated the effects of photoperiods on the serum biochemistry and histological parameters of
Heterobranchus bidorsalis juveniles exposed to artificial photoperiods for 84 days. Fish were randomly allocated into five
different photoperiod treatments at 10 fish/m2 as follows; PD1: 24 hours light (24L: 0D), PD2: 18 hours light and 6 hours
darkness (18L: 6D), PD3:12 hours light and 12 hours darkness (12L: 12D), PD4: 6 hours light and 18 hours darkness (6L:
18D) and PD5:24 hours darkness (0L: 24D) while PD3 served as the control experiment. Fish were fed twice daily with
45% crude protein commercial feed at 3% body weight. At the end of the experiment, blood samples were collected at
random across treatments and in triplicates from the caudal vein with syringes and put separately in 1ml plain tubes and
allowed to clot then centrifuged at 3000rpm for 15minutes to obtain serum for determination of serum biochemical
parameters. There were increases across treatments in the aspartate amino transferase (AST) from 25 IU in PD3 to 35 IU
in PD1 and 40 IU in PD2 as well as alanine amino transferase (ALT) from 17 IU in PD3 to 21 IU in PD1 and 32 IU in PD2. Fish
were also selected at random and dissected for histopathological observations of some organs which revealed that fish in
PD1 showed neuronal swelling and chromatolysis of the brain tissue, necrotic neurons in PD2 and PD3, spongiform
vacuole in PD4 and PD5. The liver of the fish kept under PD1 showed autolysis, PD2 contained larger cytoplasmic vacuoles
while PD3, PD4 and PD5 were normal without any visible lesions. The above suggested that fish in PD1 and PD2 were
stressed. Therefore, culturing fish species such as Heterobranchus bidorsalis under extended period of darkness could
minimize stress and enhance the health of the fish

Additional Files

Published

31-07-2023

How to Cite

Fawole, W., Ajani, E., Olawepo, K., Idowu, C., & Oyedele, O. (2023). SERUM BIOCHEMICAL AND HISTOLOGICAL ANALYSIS OF Heterobranchus bidorsalis JUVENILES EXPOSED TO VARYING PHOTOPERIOD REGIMES. NABDA JOURNAL OF BIOTECHNOLOGY RESEARCH, 2(1), 25–32. Retrieved from https://journals.nabda.gov.ng/njbr/article/view/82